Multicolor flow cytometry is a powerful tool enabled by development of advanced flow cytometers. Robust assays can detect and monitor multiple analytes to produce a tremendous amount of data …

There are 2 basic methods for staining. One is staining in 12x75mm tubes, the other is staining in 96-well round bottom plates. Generally the method is the same for both with the only difference being …

Clinically Optimized Multicolor Immunofluorescence Panel (COMIP) is a special peer-reviewed Cytometry Part B publication type that reports on newly designed and optimized multicolor panels …

This unit covers the tools needed by the beginning immunologist to plan and run multicolor experiments, with information on fluorochromes and their characteristics, spectral spillover, …

In this manuscript, we describe the basic aspects of multicolor flow cytometry, including the technical hurdles and artefacts that may occur, and provide some suggestions for how to best overcome these …

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The design of multicolor panels often requires tandem dyes consisting of two different, covalently attached fluorophores. One fluorophore (donor) is excited at a particular laser wavelength, while the …